FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_CM550273_1_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_CM550273_1_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3600533
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA64750.1798344856164351No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC64200.17830693400116038No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT58420.1622537552079095No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT54950.1526162931988125No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG54300.1508110049262151No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG51910.14417309881620305No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA51330.14256222620373152No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC51170.1421178475520152No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC49870.1385072710068204No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA49000.13609096208811305No Hit
TTCTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC48170.13378574783233482No Hit
TCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT47330.1314527599108243No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT45640.12675901040207102No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTACC43680.12131537191854651No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA43570.12100986159549156No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG42780.11881574200264239No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG39300.10915050632781315No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC38920.10809510702998695No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT37730.10479004080784705No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA36300.10081840660813274No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCC6650.082.8443145
TACGCAA406.670662E-572.488754145
CGACGAA12950.065.491776145
CGAAAGC17400.063.7985883
GCAAAAG63750.057.0198973
TACGACG805.847178E-754.4164473
TTACGAC805.8477417E-754.4156882
GTCGCCC6650.052.322712145
GCAGGGG8450.051.4949079
ACGACGG858.8845445E-751.2154774
AGCGAAA21550.051.1758651
AGGGTGT20200.050.621096
GAGCGAA13850.047.1478231
ATTACGA951.9122272E-645.8243751
CGACGGG951.9127765E-645.8224645
TAAACTA5850.044.6475265
TACACAA4400.044.481735145
ACGATAA10450.043.70136145
GCAGGGT31250.043.165099
CAAAAGC89450.043.0708124
Produced by FastQC (version 0.11.5)