FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_BHHS1178_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_BHHS1178_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2096979
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT42310.20176644592053614No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT39680.18922459404695993No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG39550.18860465460073753No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG36540.17425067203820352No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA36200.1726292919480834No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC33650.1604689412721825No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC29580.14106006784045047No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT28580.13629130286950894No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG28340.135146799276483No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC27520.13123641200031094No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA27350.13042572195525087No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT26050.12422632749302688No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC25740.122748010352035No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG24840.11845612187818763No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG21790.10391138871681596No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGACTGCTTTCTTTGGCATATCC21680.1033868245700124No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA21650.10324376162088413No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA21360.1018608197793111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC10950.076.207953
AGCGAAA13100.064.255991
TGACGCC4250.061.40229145
CGACGAA6400.057.764526145
GCGAAAG14700.057.260772
AACGCTC400.00605550354.36661145
GCAAAAG34050.054.129393
GAGCGAA6650.052.377711
ACGATAA5800.047.492676145
GTCGCCC3850.047.07066145
CAAAAGC47600.040.5500344
GACGCTC1303.705445E-739.032436145
AGGGTGT10600.038.9956446
TCCAACT18100.038.0856672
CAACTCT18000.037.8941274
TATATTC4050.037.625373
AGCAAAA52300.035.657262
GTACTGA5300.035.5749746
TAGAAAC23750.034.8303452
TACTGAT5950.034.124597
Produced by FastQC (version 0.11.5)