Basic Statistics
Measure | Value |
---|---|
Filename | HT5CKBCX2_l01_n02_BHE1244_FLUA_REP2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1831935 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA | 3514 | 0.19181903288053342 | No Hit |
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC | 3135 | 0.17113052591931482 | No Hit |
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC | 2793 | 0.1524617412735714 | No Hit |
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA | 2079 | 0.11348655929386141 | No Hit |
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC | 2012 | 0.10982922429016313 | No Hit |
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA | 1962 | 0.10709986980979129 | No Hit |
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC | 1953 | 0.10660858600332435 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGAATAA | 390 | 0.0 | 92.94119 | 145 |
GAGCGAA | 795 | 0.0 | 91.25484 | 1 |
GAGCAAA | 3600 | 0.0 | 85.444954 | 1 |
GACGAAA | 910 | 0.0 | 72.49413 | 145 |
AGCAAAA | 4550 | 0.0 | 67.28223 | 2 |
AGCGAAA | 1340 | 0.0 | 63.8852 | 1 |
GCGAAAG | 1345 | 0.0 | 63.64423 | 2 |
GCAAAAG | 4850 | 0.0 | 63.12389 | 3 |
CAAAAGC | 4870 | 0.0 | 62.715683 | 4 |
CGAAAGC | 1475 | 0.0 | 57.546234 | 3 |
TGACGCC | 450 | 0.0 | 56.384327 | 145 |
AAAGCAG | 7000 | 0.0 | 53.87043 | 6 |
AAAAGCA | 6015 | 0.0 | 50.775898 | 5 |
TTACGAC | 165 | 2.7284841E-11 | 43.96584 | 2 |
CGTGTAG | 1535 | 0.0 | 43.95272 | 5 |
TCGTGTA | 1545 | 0.0 | 43.66943 | 4 |
TATCGTG | 1545 | 0.0 | 43.66704 | 2 |
ATATCGT | 1515 | 0.0 | 43.576443 | 1 |
CGACGGG | 170 | 3.8198777E-11 | 42.673893 | 5 |
ATCGTGT | 1605 | 0.0 | 42.036926 | 3 |