FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_BHE1244_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_BHE1244_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1831935
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA35140.19181903288053342No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC31350.17113052591931482No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC27930.1524617412735714No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA20790.11348655929386141No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC20120.10982922429016313No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA19620.10709986980979129No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC19530.10660858600332435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAA3900.092.94119145
GAGCGAA7950.091.254841
GAGCAAA36000.085.4449541
GACGAAA9100.072.49413145
AGCAAAA45500.067.282232
AGCGAAA13400.063.88521
GCGAAAG13450.063.644232
GCAAAAG48500.063.123893
CAAAAGC48700.062.7156834
CGAAAGC14750.057.5462343
TGACGCC4500.056.384327145
AAAGCAG70000.053.870436
AAAAGCA60150.050.7758985
TTACGAC1652.7284841E-1143.965842
CGTGTAG15350.043.952725
TCGTGTA15450.043.669434
TATCGTG15450.043.667042
ATATCGT15150.043.5764431
CGACGGG1703.8198777E-1142.6738935
ATCGTGT16050.042.0369263