FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_BHE1201_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_BHE1201_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2545932
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTATTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT31260.1227841120658368No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT27080.1063657631075771No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA25880.1016523614927657No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT25630.10067040282301334No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC14450.065.773243
TGACGCC6250.063.7913145
GCAAAAG48550.056.636413
GCGAAAG17100.055.1538622
AGCGAAA17950.052.5421181
CGACGAA14500.049.493256145
GAGCAAA58850.048.571161
AGCAAAA58250.048.4487272
GAGCGAA11200.047.9338571
TACTCCC12700.046.804646145
CAAAAGC66400.043.3778654
GTCGCCC5300.042.399883145
AAGCAGG82750.040.6638037
ATATCGT11550.039.571931
TATCGTG11600.039.401362
CATACCG1701.8262654E-938.392968
CGTGTAG12150.037.617025
TATATTC5600.037.5712553
TAGAAAC20400.037.341162
AAAGCAG91550.036.123526
Produced by FastQC (version 0.11.5)