FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_BHE1171_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_BHE1171_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981298
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA18430.18781246879133556No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC15550.1584635859850932No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC12600.12840136227731025No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC10730.10934496962186817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4450.099.433251
CTACGCC353.4388864E-582.84917145
GAGCAAA22450.075.2837751
TACGCGG955.456968E-1268.6776145
AGCGAAA7550.062.4493941
CAAAAGC27350.061.2624474
AGCAAAA27250.061.221082
CGAAAGC8050.057.6665273
GCAAAAG29300.056.9376953
GCGAAAG8500.055.4669272
TGACGCC2900.054.994705145
ACGATCC2800.054.369762145
TATCGTG7400.053.9101642
AAAGCAG39750.053.2663046
ATATCGT7050.052.4738461
ATCGTGT7500.049.3229033
AAAAGCA35000.047.6625565
TCGTGTA7850.047.1237874
TTACGCC1850.047.0225145
AAGCAGG48100.044.4717067
Produced by FastQC (version 0.11.5)