FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_BHE1165_2_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_BHE1165_2_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2130051
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC32360.1519212450781695No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA32270.1514987199836999No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC30600.14365853211965346No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA23030.10811947695149084No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA22040.10347170091232556No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC21850.1025797034906676No Hit
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC21800.10234496732707339No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA33750.090.9227751
GAGCGAA8000.088.867341
AGCGAAA13800.065.710841
TGACGCC5900.065.11838145
AGCAAAA48050.063.4076352
CGAAAGC15350.060.0179333
GCGAAAG15250.059.9358062
CAAAAGC52500.057.204044
GCAAAAG55250.055.0132643
AAAGCAG68000.054.8172766
CAGTACG553.2148743E-452.743729
CGACGAA8700.052.492954145
TACGACG2200.049.460023
ACGACGG2200.049.460024
CGACGGG2350.046.2997325
AAAAGCA66650.045.7092635
TTACGAC2400.045.3383452
ATTACGA2450.044.415161
GCCGACC4250.039.230026145
AAGCAGG97550.038.4331677
Produced by FastQC (version 0.11.5)