Basic Statistics
Measure | Value |
---|---|
Filename | HT5CKBCX2_l01_n02_BHE1165_1_FLUA_REP2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2092761 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA | 5047 | 0.24116466237664022 | No Hit |
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC | 4427 | 0.21153872802484375 | No Hit |
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC | 3407 | 0.16279928763963014 | No Hit |
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA | 3081 | 0.1472217802223952 | No Hit |
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC | 2892 | 0.13819064862160563 | No Hit |
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA | 2778 | 0.13274329940208174 | No Hit |
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC | 2601 | 0.12428557298229469 | No Hit |
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC | 2437 | 0.11644903550859367 | No Hit |
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC | 2387 | 0.11405984725441654 | No Hit |
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT | 2373 | 0.11339087454324694 | No Hit |
AAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGC | 2354 | 0.11248298300665964 | No Hit |
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC | 2312 | 0.11047606487315084 | No Hit |
GAGTGAGACCCTGTCTCAAAAACAAGAAAGCTAAATTTGTGAACAGTTAA | 2303 | 0.11004601098739894 | No Hit |
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA | 2225 | 0.10631887731088262 | No Hit |
GAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTGCAGTGAGCTGA | 2195 | 0.10488536435837634 | No Hit |
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT | 2115 | 0.10106266315169292 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GAGCGAA | 900 | 0.0 | 94.31249 | 1 |
GAGCAAA | 3780 | 0.0 | 91.93262 | 1 |
AGCAAAA | 4820 | 0.0 | 72.09481 | 2 |
CAAAAGC | 5235 | 0.0 | 65.96697 | 4 |
AGCGAAA | 1405 | 0.0 | 65.57726 | 1 |
GCAAAAG | 5815 | 0.0 | 60.01111 | 3 |
AAAGCAG | 7240 | 0.0 | 59.90082 | 6 |
GCGAAAG | 1570 | 0.0 | 59.60814 | 2 |
CGAAAGC | 1670 | 0.0 | 56.91033 | 3 |
AAAAGCA | 6710 | 0.0 | 52.004154 | 5 |
TGACGCC | 600 | 0.0 | 47.11907 | 145 |
TTACGAC | 240 | 0.0 | 45.341457 | 2 |
ATATCGT | 2035 | 0.0 | 45.275703 | 1 |
ATCGTGT | 2095 | 0.0 | 44.32637 | 3 |
TACGACG | 250 | 0.0 | 43.529884 | 3 |
CGTGTAG | 2140 | 0.0 | 43.0532 | 5 |
TATCGTG | 2175 | 0.0 | 43.027485 | 2 |
ATTACGA | 255 | 0.0 | 42.675335 | 1 |
TCGTGTA | 2160 | 0.0 | 42.6566 | 4 |
CGCAGTC | 800 | 0.0 | 42.62097 | 5 |