FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_BHE1039_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_BHE1039_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2585268
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG118670.459023977398088No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA69010.26693557495779935No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT67540.2612495106890272No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA51800.20036607423292285No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA44080.1705045666445413No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA41200.15936452236286527No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT40850.15801069753696714No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT40520.15673423412969179No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGACTCCAACACTGTGTCAAG40180.15541909001310503No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG39530.15290484390786566No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC38780.15000379070951253No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT38210.14779899027876414No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATATTATTTGGGTCTCCATTCCC36120.13971472203268676No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT32600.12609911235508273No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA29420.11379864679406544No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG29160.1127929483519697No Hit
GATCCTCATGATCTCAGAGAACTCCTCTTTCTTAATCCTTCCAGACTCGA29010.11221273771229907No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC28390.10981453373499382No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC28330.10958244947912557No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC27720.10722292621113169No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAATTACGGAATCTGATGTCACA27080.1047473608152037No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA26470.1023878375472098No Hit
TTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGGACAAAGCGTCTACG26040.10072456704682067No Hit
CTTTTAAGGCCATGTTTGAAGAGTCGATAGATGCATTTGAAAAAAAGACG25960.10041512137232969No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA11100.090.194121
AGTAGAA13800.086.21581
TGACGCC4650.077.947655145
CAAAAGC66850.074.882134
TCTTACC5800.069.991615145
GCAAAAG73950.067.00593
AGCGAAA18900.060.648121
AAGCAGG98950.060.6040577
CGAAAGC19650.057.9652373
AGCGTAA651.094909E-555.76255145
GCGTCAG553.2095757E-452.7617761
AGCAGGT63250.051.2458848
GCAGGGT23550.049.884979
TACGCGG2200.049.4259145
GCGAAAG23200.048.7809562
TTACGCC3300.048.32754145
GCAGGGG5300.046.5209589
AAAGCAG128950.046.2814876
GCAGGTA59950.045.6034559
TTAATCC43350.045.3341839
Produced by FastQC (version 0.11.5)