FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_BHE1015_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_BHE1015_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3713802
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA74160.1996875439239895No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC62060.16710637777673662No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT59300.15967464070513182No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT57400.15455858982250534No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT56610.15243138971867642No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG56470.15205441754837765No Hit
TCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT53680.14454190072599454No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA52170.14047598660348612No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC51740.139318143508997No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC50790.13676011806768373No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG48620.13091704942805243No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA45790.12329682627129826No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG44650.12022719574172236No Hit
TTCTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC42520.11449183343646215No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA42520.11449183343646215No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA41560.11190688141155614No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTACC41170.11085674465143808No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC41050.11053362564832482No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG38370.1033173012454622No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT37920.1021056049837875No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC3350.093.04799145
CGCCACC353.440655E-582.84672145
TGACGCC5850.081.784584145
GCAGGGG8650.079.6485759
ACGCCCC300.001937124472.490875145
CGACTCC955.456968E-1268.67557145
AGCGTAA651.4003126E-766.91466145
GCAGGGT21100.066.679119
CTTATAA2550.062.54115145
AGGGTGT16950.059.471626
CGACGAA10450.055.495407145
CGTACGA400.00605518854.368156145
GTCGGGG400.00605518854.368156145
CGAAAGC20150.051.8474773
GCTGACC2150.050.57503145
GTCGCCC6500.050.185993145
TAAACTA5100.049.787525
GCAAAAG77700.049.5808223
ACTATTC5300.049.2569968
TTATATT9450.049.1340452
Produced by FastQC (version 0.11.5)