FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_99_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_99_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583661
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA259.3929906E-486.98782145
ACGAGCG1150.081.981299
AAACACG21650.077.418165
AACACGA14800.074.481966
TAGTAAC22050.072.394042
ACACGAG16150.068.253757
GCACGCA6500.064.7445762
GTAACAA24400.063.6414644
AGCACGC6700.062.81191
ACAACGC6600.059.334238
CACGAGC18700.058.9464238
GAAACAC29150.057.7518124
TAACAAG27050.057.671335
GTAGAAA40050.054.7133371
AACACGT10850.053.4723786
ACGAGCC5300.053.365189
ACGAGCA11700.051.4471669
TGACGCC3400.051.169304145
AACAACG7400.050.9597327
CGGCGAA2150.050.596689
Produced by FastQC (version 0.11.5)