FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_92_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_92_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1677813
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTAGGAGTGCTTTCTGAAAGGTTGATCCCGGGCAAGATAAACGAGGGT23210.13833484422876685No Hit
AGTAGAAACAACGCACTTTTTCCCAGTTTATTTGCTGACATTGATTACAA22620.13481836176022No Hit
GTAAGAAGTACAGCAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA21840.13016945273400551No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA18040.1075209215806529No Hit
GGATAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATA17260.10287201255443842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG8900.085.557647
CACGAGC9450.079.810698
AAACACG15600.077.205855
GAAACAC15450.077.0162054
AACACGA10000.076.878386
ACAACGC6950.076.1723868
CAACGCA10300.074.641439
ACGAGCC2950.068.841029
ACGAGCA5950.065.8245859
AACAACG9150.060.235437
AACACGT7250.059.021796
ACACGTG7400.058.8002367
AGCAACG4250.056.309877
TCTCGGG7900.055.971645145
GTAACAA18450.055.0552144
ACGTGCA3650.053.6515469
TAGTAAC19400.053.1071972
ACGAGCT2800.051.806389
TAACAAG21150.048.7129825
TGACGCC18950.047.815216145
Produced by FastQC (version 0.11.5)