FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_78_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_78_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2059817
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA42270.2052124047912994No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA36930.17928777168068816No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC30270.14695480229554372No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT23990.1164666569894316No Hit
TCTCTCTTCTGTCCTTCCAAGCCTACTAAGCACGTGGCATCCTCCCAGAT22960.11146621277521254No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT22840.110883636750255No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC21050.10219354437797144No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG20980.10185370836341286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA10100.091.235662
GTAACAA46750.081.640854
TAGTAAC47400.081.435842
GTAGTAA48400.080.652751
AGCACGC11700.078.758991
CACGCAC12700.074.275063
ACAACGC7250.071.023518
CGAGCTA1900.068.740355
TAACAAG56200.068.040445
AGTAACA57600.067.0181663
AAACACG24750.065.962965
CGCACTT15000.065.303335
CTCGCTC6100.064.2343443
CGGTGCG808.041752E-963.4878042
GCGGTGC5200.059.999461
GCCGACC2900.059.99073145
ACGCACT16950.059.93244
GAAACAC27950.058.4122664
AACAACG8850.058.1831057
ACGTGCA8500.055.485142
Produced by FastQC (version 0.11.5)