FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_740-5_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_740-5_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694678
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT19280.2775386581984747No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA15950.22960277999303272No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT15470.2226931038553114No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT14480.20844189682126105No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA14000.20153222068353974No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG13030.18756891682189447No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT11890.1711584359948062No Hit
GTATTTATCGTCGCTTTAAATACGGTTTGAAAAGAGGGCCTTCTACGGAA10790.1553237615125281No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC10590.15244472978847753No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC10500.15114916551265478No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC10320.14855803696100928No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG9730.1400648933750601No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC9390.13517053944417412No Hit
AAAGCAGGCAGAGAGGCAGGCAGAGAGAGGGAGGCAGAGAGAGGGAGAAG9280.13358707199594633No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA9130.1314277982029084No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA9080.13070804027189575No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT9060.13042013709949069No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC8910.12826086330645275No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG8690.12509392840999717No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC8480.12207094509974405No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT8290.11933586496189602No Hit
ATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTCACT8230.11847215544468084No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC8200.11804030068607327No Hit
AGCGAAAGCAGGGAGAGGGGGGTTTAGATAATGTCCTGACCCTGGGCCAG8160.11746449434126315No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA8060.11602497847923786No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC7950.11444151103101004No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT7950.11444151103101004No Hit
AGCGAAAGCAGGCAGAGAGGCAGGCAGAGAGAGGGAGGCAGAGAGAGGGA7720.1111306245483519No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG7710.11098667296214938No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT7650.1101229634449342No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT7530.10839554441050385No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT7510.10810764123809881No Hit
GAATGGGAGACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAG7490.10781973806569375No Hit
CTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTTGA7370.1060923190312634No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTA7360.10594836744506088No Hit
GTCCTGACCCTGGGCCAGTGTCTTCAGTGAGCTGCTCCTTGCTTCTGTTC7360.10594836744506088No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG7310.10522860951404825No Hit
ATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGTTCCTG7190.1035011904796179No Hit
CCTTAGAAGATGGCCATGTGGGGTGCCTGGGTGGCTCAGTGGATTAAGCC7160.10306933572101032No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA7130.10263748096240272No Hit
GGCAGAGAGGCAGGCAGAGAGAGGGAGGCAGAGAGAGGGAGAAGCAGGCT7110.10234957778999768No Hit
GAAAATATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACC7040.10134191668657996No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA9550.0115.442661
GAGCGAA2000.0108.79711
GCGAAAG11150.098.869772
CGAAAGC11150.098.219313
CAGGTAC300.00193272272.5261843
GTGCGTA300.00193272272.5261847
GCAGGGT4750.070.235888
TCTGACC1450.069.98999145
GAAAGCA16250.068.286194
CCTTACC1300.066.913506145
AGACGAA554.063746E-665.899666145
GATAGCA350.00356094862.16536
TACACAA1800.056.38083145
TAGAAAC7850.056.357922
GTAGAAA7900.056.0052571
TAACGGG400.00605353554.367226145
ACAAGGC2600.052.99998
CAGGGTG6100.048.7506189
ACAATCG450.00962416748.350793
CGAGGGA450.00964335248.326424145
Produced by FastQC (version 0.11.5)