FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_67_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_67_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2607524
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA62370.2391924292930765No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC52990.20321960603238934No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA44200.16950946568468783No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC44190.16947111512684065No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT40110.15382408752517715No Hit
TCTCTCTTCTGTCCTTCCAAGCCTACTAAGCACGTGGCATCCTCCCAGAT33830.12973993719712645No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA32870.12605828364379387No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG31400.12042075164025337No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA31150.119461987694073No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG30600.1173527070124762No Hit
CTGCATATCTGCTCCTTTGTATAAAGGATAAACAAAAACTTTAAAGTACT27680.10615434412108958No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC27180.10423681622872885No Hit
AACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGC26120.10017165709692412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG40200.081.942395
TAGTAAC48200.081.441492
GTAGTAA51700.077.053791
AACACGA29550.075.59286
ACACGAG31100.071.822557
GTAACAA56550.069.16084
AACACGT16450.066.132136
ACGAGCC15050.066.019359
GCACGCA11150.064.425212
CACGAGC34750.064.2785958
TAACAAG61600.063.722885
AGCACGC11550.061.5681761
GAAACAC53700.061.4810074
TGACGCC7550.061.447407145
ACAACGC9650.057.867398
CGACGAA12000.057.386925145
GCCGACC4200.056.95544145
ACACGTG19450.055.9296577
AGTAACA70900.055.5721783
TAGAAAC83300.053.3091742
Produced by FastQC (version 0.11.5)