FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_562-5_Gr9_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_562-5_Gr9_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2607059
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG42520.16309565683016763No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA31050.11909972118007302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6750.0118.226771
GCGAAAG13100.069.222772
CGAAAGC13550.067.9946443
AGCGAAA13650.066.436131
TGACGCC4100.061.880356145
TTACGTG2450.056.2597472
TACGTGT2600.053.013993
GCAGGTA10900.052.5606659
GTTACGT2650.052.0157241
ACTCCGA4050.051.9481281
CCGCTCT5400.048.3636444
GACGAAA14300.048.15664145
CCCGCTC5450.047.919943
GGTTATA2900.047.531611
TCCGAGG4850.044.873483
GTTATGT8500.043.528951
CTCCGAG5200.043.2482572
AACGGGT5000.042.0763663
CAAGGTA5450.041.250149
ACGGGTT5150.040.850844
Produced by FastQC (version 0.11.5)