FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_561-5_Gr9_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_561-5_Gr9_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573635
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAAGCAGGCGGCAAGGATTGTTATGCCATAGATTAGGGGCCGATGT16370.28537310310563335No Hit
CTATGATTCTGATCGAGACCCTAGGTATCCATCATAGGAATCGTGGTTTA13200.23011148203997317No Hit
AAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGA12510.21808292729697457No Hit
GTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGAGCTTCTCGCAAAG12270.21389908216897505No Hit
GGTATAGGGAGGGAGGCGATGCCATCATTGGCATTCCGATAGCATCAGCC10070.17554716849564617No Hit
GTCATAGAGTAGTGGTAACACAAAGCAGTTAATTTGTGATTCGTTGTTGT9980.17397822657264636No Hit
TTTTTTATCAGTGACTTTCAAACACAAATGCAATTAACACCACTCAAACA9240.1610780374279812No Hit
AGCGAAAGCAGGGTGCTGCAAAAGTCATATCTATGATTCTGATCGAGACC8960.15619688477864843No Hit
AGTAGAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT8470.1476548676423161No Hit
TTCTAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAAATT7770.1354519860189842No Hit
GTTCTGAGTTTGCCATTGAAAAATGCGTGGTACCTTTGTCTTCTTAGTCA7580.13213977529265125No Hit
GATTAGGGGCCGATGTTTCTGTCATAGAGTAGTGGTAACACAAAGCAGTT7570.13196544841231794No Hit
TATCAAGACGGTGCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCT7420.12935054520731823No Hit
TTCCATAGGCAGTCAAAAATGTCACAAACAACTCAAAGCCACAAATCCGG7420.12935054520731823No Hit
AAAGCAGGGTGCTGCAAAAGTCATATCTATGATTCTGATCGAGACCCTAG7360.12830458392531838No Hit
GTGTTGGGAAAGTGGGAGGAGAATGAGTGCACCACTGCTGCTGCTGCTGC7290.1270842957629852No Hit
CCCTAGGTATCCATCATAGGAATCGTGGTTTAAGCATTTTTTATCAGTGA6530.1138354528576534No Hit
ATATAGAACAGCAAACGCAATTACCCCATCAACAAGTTAAGCTGTTGAAA6500.11331247221665344No Hit
AAAGCAGGCAGGCTGGGCCACTGCGAGGCCCTCTCTTGTGGTGTTTATGC6390.111394876532987No Hit
AGCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTAT6110.10651372388365424No Hit
TCCTTAAACTTTAATAACGGCGTTAAGGGCAATAAGATGAGGGTTATGGG6040.10529343572132105No Hit
GGCTGGGCCACTGCGAGGCCCTCTCTTGTGGTGTTTATGCAAAAGCCGGT6000.10459612819998781No Hit
GAGTAGTGGTAACACAAAGCAGTTAATTTGTGATTCGTTGTTGTGCTTGT5810.10128391747365485No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA20200.0123.2168351
GAGCGAA3450.0113.580031
GCGAAAG24500.0102.4795762
CGAAAGC26050.096.381943
GAAAGCA27950.089.830054
AGCAGGT6050.065.927927
GTAGAAA2950.063.9556122
TAGGGAG1850.062.7589345
TATAGGG1850.062.7589343
ATAGGGA2000.058.0520174
ACCGGGG502.0066819E-458.016578
AGTAGAA3350.056.319121
AAGCAGG52150.055.3465846
AAAGCAG53650.054.5082975
GGTTACC1206.002665E-1154.3905378
ACGAATA400.00605526354.362087145
GGGGTTA1101.6698323E-952.742346
AGGACTA701.6861004E-551.832165
GCAGGCG6650.050.1647078
CGACGAA4800.049.83191145
Produced by FastQC (version 0.11.5)