FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_561-3_Gr9_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_561-3_Gr9_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915692
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG32000.16704146595590524No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA26040.1359299929216179No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC24290.12679491275215432No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA23940.12496789671826161No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA22190.11583281654879803No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC20130.10507952217788663No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG19830.10351350843455001No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA19690.10278270202099295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4450.0117.3909151
AGCGAAA7800.066.039392
GTCGTCT2100.065.58593145
GCAGGTA8750.062.9904449
TTACGTG3000.062.876942
TACGTGT3050.061.8477863
ACTCCGA5750.059.3050961
CGAAAGC8850.058.205734
GTTACGT3250.058.0432851
GCGAAAG9100.056.606673
TCCGAGG6200.054.999263
CTCCGAG6450.052.8661232
TGACGCC4550.052.57496145
GGTTATA3050.052.334111
GTAACTC1900.049.6409873
GTTATGT9350.046.558791
CGAGGTG7650.046.4664965
GAGCAAA57200.046.424481
AAGCAGG71450.044.760427
AGTCGGC1151.4066791E-744.1576045
Produced by FastQC (version 0.11.5)