FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_55_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_55_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919860
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT28400.3087426347487661No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA24240.2635183614897919No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC24170.2627573761224534No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA16660.18111451742656492No Hit
GATTAAGAATGGATGCACGATTAGATTATGAATCAGGGAGAATGTCAAAG16250.17665731741786794No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT13780.149805405170352No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC13270.1442610832083143No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC13140.14284782466897136No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT12800.1391516100276129No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT12340.13415084904224556No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT11780.1280629661035375No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA11490.1249103124388494No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC11210.12186637096949536No Hit
CCCCATAAACATCTTCGAAGCTTATATGTACCCAATCTCACCAAGGTGAG10760.11697432217946209No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA10640.11566977583545324No Hit
TTTGTGTATCGCATGTATTCAATGATGACCAATAACCCCATAAACATCTT10640.11566977583545324No Hit
CTCTACAGCAGAGGGGCCCAGCATTTTCTTCAGCTTTCTTTCAAGCGCCA10620.11545235144478508No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG10520.11436522949144436No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT10260.11153871241275845No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC203.8679937E-4108.7356145
CCCGAAA301.6004422E-596.65388145
CGTCACC353.4393863E-582.846176145
ACACGAG3850.077.226977
GAAACAC5500.076.540134
AACACGT1550.074.865436
AAACACG5800.072.573265
CACGAGC4200.070.791388
ACGAGCA1650.070.328149
GAGCAGA3100.067.8985061
TAGAAAC7100.066.4439242
CTTACGG451.1945897E-464.43591145
AGAAGCG2950.063.9628755
TGACGCC2650.062.9162145
AACACGA4850.062.8059276
ACGAGCC2900.062.522329
AGGTGAC350.003562905662.1582877
ACGTGCC1300.061.368069
ACACGTG1950.059.501957
GCCGACC2000.057.992325145
Produced by FastQC (version 0.11.5)