FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_528-3_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_528-3_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156821
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8550.067.8913961
AGCGAAA14350.065.7328341
GCGAAAG14800.063.7327162
TGACGCC7050.061.692158145
CGAAAGC15800.061.078093
GAGCAAA53050.047.1872371
GACGAAA10650.046.283596145
GCCGACC3400.042.640163145
CAAAAGC63250.038.430424
GCAAAAG65050.036.4746633
GTAACGG4450.032.6107561
AGCAAAA75100.031.7860642
CGGGTTG4800.030.2314835
AACGGGT4850.029.9212073
AAAGCAG102000.029.2213866
TACGAAC1000.006066345629.0094349
ACGGGTT5150.028.1782264
GTTACGT2559.422365E-825.6090341
GTGCGAT2859.8752935E-925.4468739
CGAAGCG4001.8189894E-1225.3944455
Produced by FastQC (version 0.11.5)