FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_427-3_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_427-3_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460201
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG58900.23941133265127523No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA43370.17628640911860452No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA42350.17214040641394748No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA40700.16543363733288458No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT38280.15559704268065902No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT38120.15494668931522262No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA31500.1280383188202915No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC29600.12031537260573424No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG29520.11999019592301606No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC29350.11929919547223986No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT28890.1174294295466102No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC28370.1153157811089419No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT28350.11523448693826237No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC27650.11238919096447811No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT27360.11121042548962463No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT25700.10446300932322196No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC25700.10446300932322196No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5950.0128.03921
AGCGAAA13950.079.056931
GCGAAAG15350.072.791882
TTACGTG607.9940946E-872.555552
CGGGCGG300.001937068972.49066145
CGAAAGC15600.072.090453
GCAGGTA12250.060.976129
GTAGAAA17900.058.368711
GTTACGT753.7436075E-758.0444371
TGACGCC4800.057.38844145
CACCTAC5350.055.6033133
CGATTAA400.00605501854.367992145
CAATTCG7400.050.9601029
TACGTGT901.3173994E-648.3703653
GGTCGTT4100.047.7552227
TAGAAAC22300.047.1773722
AAGCAGG79650.046.614947
CCACCTA6400.046.4808962
CCTCGCC1002.7382048E-643.494396145
GAGCAAA72000.041.51791
Produced by FastQC (version 0.11.5)