FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_286-5_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_286-5_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341626
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAAGCAGGACGAAGCTGCCGTTGCGTCCGCCAAGCGCACCCTGGAC9510.2783745967812754No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT7730.22627083418709348No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC6750.1975844929835551No Hit
GTTTAATACTGTGAACAGCAGCATAGAGTTTAATACTGTGAACAGCAGCA5570.16304379643235584No Hit
ACATGCGGCCAAGCCCGCTGCTTGCCGCGACGGCGCTCAGGGATTCCGGA5450.1595311832237593No Hit
AGCGAAAGCAGGGGGATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCG5280.15455498117824756No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA5250.15367682787609843No Hit
CTATAAGCCAACGCGGTGCTGCCGGCGGTAACGGTTTGTCAGGTGCGCCG5170.15133508573703405No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCTTTC5120.14987149690011886No Hit
AAGCAGGACGAAGCTGCCGTTGCGTCCGCCAAGCGCACCCTGGACGGTTT5120.14987149690011886No Hit
GCCCTGGACACTGCTTCGAAACGCGAGCATCTCGCCGTGGCTGACGGCTT4890.14313898825030882No Hit
ATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGATTATCCCTCAGGTC4740.13874822173956314No Hit
TCACTATGCTGCTATTCACTCTCACTATGCTGCTGTTCACAGTATTAAAC4700.13757735067003096No Hit
TTAATACTGTGAACAGCAGCATAGAGTTTAATACTGTGAACAGCAGCATA4610.13494289076358357No Hit
GGCTTATAGTGCGGAGTGCCCATTCAACGAGGAGAGCGTTCATGAAACTG4490.131430277554987No Hit
AGCGAAAGCAGGGAGCCATGGCTGAGGGCCTCGATGACGACCAGAAGACG4470.13084484202022095No Hit
GTTTTAGTGGTGTTCACTATGCTGCTATTCACTCTCACTATGCTGCTGTT4420.12938125318330573No Hit
GGACGAAGCTGCCGTTGCGTCCGCCAAGCGCACCCTGGACGGTTTCCATC4400.12879581764853962No Hit
GGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGG4370.12791766434639049No Hit
CTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTTGA4190.1226487445334957No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC4080.11942884909228221No Hit
GTCAAAGACGGGAAGGCGATTATCCCTCAGGTCGAATCCGGCATCCTCGA4050.11855069579013308No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC4050.11855069579013308No Hit
TCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGATTATCCCTCAGGTCGA4020.11767254248798394No Hit
AGCGAAAGCAGGTGTACAGGTGTACAGGTGTGGATGTTTAATACTGTGAA3980.11650167141845176No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC3970.1162089536510687No Hit
ATGCTGCTGTTCACTCACTATGCTGCTGTTCACTCACTATGCTGCTGTTC3940.11533080034891957No Hit
GTCTTCGGGCGTACCGACGCGTCCGAGCGGATGAAACGAATCGAGCGCTT3920.11474536481415348No Hit
GTGTACAGGTGTGGATGTTTAATACTGTGAACAGCAGCATAGAGTTTAAT3880.11357449374462131No Hit
CACTATGCTGCTGTTCACAGTATTAAACTCACACTATGCTGCTGTTCACT3870.11328177597723828No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG3760.11006188053602477No Hit
TCCCTGAGCGCCGTCGCGGCAAGCAGCGGGCTTGGCCGCATGTCGCTCGA3740.1094764450012587No Hit
GGTGTACAGGTGTGGATGTTTAATACTGTGAACAGCAGCATAGAGTTTAA3730.10918372723387564No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA3590.10508567849051302No Hit
GTGTACAGGTGTACAGGTGTGGATGTTTAATACTGTGAACAGCAGCATAG3580.10479296072312998No Hit
AGTAGAAACAAGGTGTTTTAGTGGTGTTCACTATGCTGCTATTCACTCTC3530.10332937188621476No Hit
AAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCGAACGT3480.10186578304929952No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACATG100.0070911646144.98405145
TGACTCC100.0070911646144.98405145
AGCGAAA8850.0113.95761
GCGAAAG9750.0103.4384462
CGAAAGC10200.098.163653
TACTGAT950.091.595697
GGAAGCG259.352813E-487.0668955
ACTGATC950.083.9627158
CCGACGG353.435906E-582.84803145
GAGCGAA2800.077.73831
GCAGGGC1050.075.966268
GAAAGCA14400.072.555744
AAAGCAG16700.072.555745
GAAGCGC300.001933245172.513256
GGCCCCG300.001933245172.513259
ACATAGC300.001935492372.49203145
ATACCAA300.001935492372.49203145
AAGCAGG17750.067.4066856
CAGGTAC1300.066.974533
CAGGACG1950.066.935319
Produced by FastQC (version 0.11.5)