FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_199-7_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_199-7_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2972872
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA64080.21554913901439418No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA58820.19785581081190176No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA48690.1637810171443641No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC45290.15234426507431198No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG42700.14363215099741933No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG41600.13993202532769658No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA39540.13300269907348855No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT33220.11174379522562693No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC30810.10363715625832529No Hit
GATATGTTCCTTCTATTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCC30780.10353624374006011No Hit
AGTCAGTGGAGCTCTGTGACTCCTGATCTTGTGGTTTTGAGTTTCAACCC30360.10212346848434779No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9350.0103.9724661
CGAATAA6600.091.16242145
AGCGAAA14300.064.9347462
CGAAAGC14450.064.763814
ACTCCGA10200.060.4566271
GCGAAAG16350.060.3436663
TTACGTG4950.060.0871852
GTCGTCT2950.058.975403145
GTTACGT5400.057.7696651
TACGTGT5200.057.1993453
TGACGCC5050.055.98284145
TCCGAGG11150.055.954383
CTCCGAG12450.050.6935962
AACGGGT11650.048.5712433
CGCGCTT450.00961712248.3636743
GGTGCGA8100.047.448888
TATGTGT18150.046.7648743
AGCTACT10400.046.7171758
GAGCAAA76950.046.668271
ACGGGTT12500.046.4291234
Produced by FastQC (version 0.11.5)