FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_156-7_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_156-7_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413116
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA72340.2997783778318158No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA63010.2611146749679667No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC57580.23861264854238254No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG50470.2091486691895458No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA46010.19066634177553007No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG43900.1819224604204688No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA43180.17893876630878913No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA36390.15080087322781002No Hit
GATATGTTCCTTCTATTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCC35090.14541364774838839No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT34260.14197411148075767No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA34140.14147682912881104No Hit
AGTCAGTGGAGCTCTGTGACTCCTGATCTTGTGGTTTTGAGTTTCAACCC33900.14048226442491785No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC33610.13928049874104684No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG32530.13480495757352734No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC31410.13016365562202564No Hit
GTAGCATGTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG30240.12531515269054616No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG29760.12332602328275971No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT29740.12324314289076863No Hit
CTTATATGGACTTGACAGATACACACAGAACCCTCCATCCAAAAGCAGCA27260.11296597428387196No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA26710.11068676350411667No Hit
CTCCCGCTCTGTGTCAAATCAATAAATGACATCTTTAAAAAATAAAGTGA26490.10977507919221455No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA25950.1075373086084548No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA25850.10712290664849927No Hit
GAGCTGGAGAGAGAGACAAACTGACAGACTGAGCAAAGTGGAAGGAGCAG24620.1020257625410465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3400.089.6218261
ACGTGTT4950.086.471344
GTTACGT5550.078.433511
TACGTGT5550.077.1230853
TTACGTG5550.077.121482
GCTACTA8600.076.735669
AGCTACT10050.065.662998
CAGCAAC16350.061.2077837
GATAGCC2500.060.893585145
ACTCCGA7950.057.4932481
GGTGCGA6950.057.3882758
TGACGCC4700.057.068455145
GTGCGAT7250.057.0152669
TATGTGT16400.056.1804543
AGCGAAA5400.055.0815932
GCGATTA6200.054.95389145
GCGAAAG6500.054.690023
CAGTGCA30150.054.3797872
CTCCGAG10700.054.2403642
TCCGAGG10250.053.0838973
Produced by FastQC (version 0.11.5)