FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_102_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_102_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2790831
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT61790.22140358911019692No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT43430.15561673207729168No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA33260.11917597303455493No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG30920.1107913736087925No Hit
GATATACAGAAGGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA30780.11028973090810587No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCGGGCACTGAAG29460.10555995687306038No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT28470.1020126263467763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC13700.094.796811
GCACGCA15600.083.2480552
GTAGTAA32500.078.581541
AAACACG25550.077.2353065
GACGAAA12850.074.46472145
TAGTAAC34250.073.292782
ACAACGC13100.070.3020258
AACACGA17750.068.637576
ACACGAG17850.067.4380957
AACACGT11150.065.6897356
CACGCAC20700.063.7902833
TGACGCC4600.061.45915145
CACGAGC19600.061.4168368
GTAACAA42950.058.954334
AACAACG15900.058.377877
CGCACTT23300.057.9155125
AGTAACA45750.054.5532723
ACACGTG14250.052.415277
CAACGCA22250.051.824339
GAAACAC39700.049.5258834
Produced by FastQC (version 0.11.5)