FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10513127
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA21200.074.549211
CGAAAGC37650.058.9014433
CGCACTT4200.058.6646355
GCAAAAG147300.052.1521953
GAGCAAA149150.052.1067431
AGCAAAA172200.045.1596452
ACGCACT6050.044.319324
TATCGTG13800.038.3378452
ATTACGA2103.783498E-1034.5225681
GCAGGTA64700.033.7206349
ATCGTGT15150.033.4853553
AGCAGGG110750.033.431338
CAAAAGC255850.030.9302884
AAAGCAG313800.030.0680186
CACGCAC7800.028.8029233
TCGTGTA18000.028.1820324
GGTAGTT13750.026.873197
AAGCAGG359650.026.3499267
GCAGGGT91900.024.7655329
AAAAGCA332350.024.1814675
Produced by FastQC (version 0.11.5)