FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_SAMPLE_6_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_SAMPLE_6_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3029787
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG53270.1758209405479659No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA51610.17034200754046405No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT44430.1466439720019922No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA36840.12159270602190846No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT36700.12113062733452878No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT35690.11779705966128973No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC35540.11730197535338292No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT33550.11073385686848614No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA32960.10878652525738608No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA32020.10568399692783685No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT31880.10522191824045718No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT31060.10251545735723336No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAG100.0070888386145.018664
CCGGGTC100.0070919874144.99713145
GAGCGAA5700.0132.334871
GCAAAAG38750.097.867443
AGCAGGC2250.096.664758
TATTAGT651.6370905E-1189.267311
CGCGTAT259.3893404E-486.99827145
AGCGAAA14200.084.277451
ATTCCGC353.4387438E-582.8555145
ATTAGTA1650.079.123291
GTCAATT4350.076.680332
GCAGGGG16650.073.15179
TGTCCTA300.00193627572.498566145
GCAGGGT20250.071.603529
TAAACTA7300.063.5698285
TTTTTAA7400.062.728381
GTCGAGG350.003567482662.141624145
CGCCGTC350.003567482662.141624145
CTATTCG7600.062.005359
AAGGGTG10600.061.5645265
Produced by FastQC (version 0.11.5)