FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_SAMPLE_4_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_SAMPLE_4_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3535459
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA61440.17378224439881781No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG59560.16846468874338522No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT53030.14999466830190933No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT44700.12643337116906178No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA43330.12255834390951784No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC41950.11865503177946625No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT40530.11463858016738421No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT38790.10971701269905831No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT37900.10719965922388011No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT37280.10544599725240769No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT37230.10530457289986957No Hit
GAGTAGAGGCTTTGGGTCCGGCATCATCACCTCAAACGCATCAATGCATG36970.10456916626667145No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA36460.10312663787078284No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGG100.007081479145.06941
AGCGGGG100.0070922687144.995548
GAGCGAA6850.0131.30371
GCAAAAG47700.098.357813
AGCAGGC2900.094.9970868
AGCGAAA17400.087.541881
GTCAATT6650.082.874522
GCAGGGG19250.073.815919
GCAGGGT25800.071.373789
ATTAGTA2100.069.080671
AAGGGTG12050.065.590035
TAAACTA8100.065.3485955
ACAAGGG14650.065.337013
AGGGTGT12250.064.519176
CAAGGGT14800.064.181224
TTTTTAA8400.063.0361181
CTATTCG8400.063.004029
GCGTCAG350.003560495362.1726041
AGCAAAA76500.061.993392
CGCACGA502.0112502E-457.998222145
Produced by FastQC (version 0.11.5)