FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_SAMPLE_3_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_SAMPLE_3_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733409
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG65450.2394445909851032No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA62190.2275180918772127No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT40980.1499226789697407No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT40610.14856905790534822No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT39180.14333749541323673No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC34520.12628918687250973No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA34010.12442338486483362No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT33730.12339902297826633No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG33070.12098445567421487No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA30170.11037499327762512No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA29810.10905795656632432No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT29600.10828968515139885No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT28960.10594828655353078No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG28560.10448491242986321No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA28210.10320446007165411No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT27820.10177767030107826No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCGG100.007092217144.9953145
GAGCGAA7950.0132.281651
GCAAAAG43150.0101.3322753
GTCAATT6250.081.216682
AGCAGGC2900.079.9974068
GCAGGGG17400.079.16419
GCAGGGT22700.078.246369
AGCGAAA16350.076.297341
AGCAAAA64850.068.098032
AGCAGGG48650.065.121228
AAGCAGG86600.062.53557
GAGCCCC1600.058.904343145
CAAAAGC75650.058.7563634
AAGGGTG10650.056.5108535
AGGGTGT10800.056.3973736
TTTTTAA7300.054.643511
TAAACTA7300.054.631515
ATCCGCC400.006052779554.373238145
ATTAGTA1500.053.1863481
ACAAGGG12650.052.162963
Produced by FastQC (version 0.11.5)