FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_SAMPLE_2_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_SAMPLE_2_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177582
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG61930.1948966226520669No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA60670.19093134339255446No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT43920.13821830561729012No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT41270.1298786309841886No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA40000.1258818812543626No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT38000.11958778719164445No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT36960.11631485827903103No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA36490.11483574617429228No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA35660.11222369713826424No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC35650.11219222666795066No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT33660.10592960307554612No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG33550.10558342790209663No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT33050.1040099043864171No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT32110.10105168017693958No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA100.0070923804144.99455145
TTACGTC100.0070923804144.99455145
GAGCGAA6200.0128.705221
GCAAAAG46750.094.152383
AGCGAAA16350.091.843351
GACCGCC259.390045E-486.99673145
AGCAGGC2350.077.124768
GTCAATT5700.075.06232
TACTAAT1550.074.83597
GCAGGGG20800.073.891469
CTACTAA1700.068.245626
TCTACTA1700.068.245625
ACTAATA1700.068.2327358
GCAGGGT23400.067.2303859
ATTAGTA1850.066.661081
TAAACTA8050.064.854545
CTATTCG8000.064.341339
TTTTTAA8050.063.981971
AGCAAAA71200.062.4345782
TATTAGT702.326633E-762.179661
Produced by FastQC (version 0.11.5)