FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_SAMPLE_1_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_SAMPLE_1_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720797
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTAGATTGCCCTGTTGAATAATGCAGTTGGGGGAGTGTTCGTGTAGA22120.3068825203212555No Hit
AGCGAAAGCAGGGCCCGCTGGAGCCAGCAGAAGACTTAAGCCAGACATGC22080.3063275790548518No Hit
ATGTATAATTGATGACTGCTAGATTGCCCTGTTGAATAATGCAGTTGGGG18900.2622097483757563No Hit
AGCGAAAGCAGGGGCTTTTGGAGGAGGGTGCTTGTGTGTGTGTGTGTGTG18780.2605449245765451No Hit
TATATGAACAGCTTGATAAACATGGTAGCCAAAAAAAAGTTTCCAAAGTT18710.2595737773603386No Hit
ATAAAAAGCCTTTTAACGAACTTCCTGAAAAGAAGAGCATATATGTTCAT16120.22364133036069794No Hit
GTTTTGAGTTGGGTGGAGAGGTTGATCATCAGCGCCAAGGACAACAAGAA15610.21656582921405057No Hit
GATAAACATGGTAGCCAAAAAAAAGTTTCCAAAGTTTTAACACAGTTTTG15530.2154559466812431No Hit
GCTTGATAAACATGGTAGCCAAAAAAAAGTTTCCAAAGTTTTAACACAGT15200.21087768123341247No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT14840.205883209835779No Hit
AGCGAAAGCAGGTGGCACGTCACACCCCACCCCACAGCTCTTCAACTATA14510.20130494438794833No Hit
ACTCAAAACTGTGTTAAAACTTTGGAAACTTTTTTTTGGCTACCATGTTT14220.1972816202065214No Hit
GTCTACTGCATCATTTTGAGCTTTCTAAGGGAAAAAAACAAGACCACAGA14100.19561679640731025No Hit
AGCGAAAGCAGGGGGTCTGGGGGGGGAAGTCCCATCATATTGCGAGGGCC13320.18479544171243775No Hit
GAATAATGCAGTTGGGGGAGTGTTCGTGTAGATTCTGCTTCTTGTTGTCC13070.18132705879741454No Hit
TTATCAAGCTGTTCATATAATAAAAAGCCTTTTAACGAACTTCCTGAAAA12940.17952349968160244No Hit
TATATGTTCATGGCACAGGGTTCCTGCTCGAGTGTCGAGTGCTTGTTTCC12590.1746677636005699No Hit
GTTCATATAATAAAAAGCCTTTTAACGAACTTCCTGAAAAGAAGAGCATA12420.17230926321835413No Hit
GTTGGGGGAGTGTTCGTGTAGATTCTGCTTCTTGTTGTCCTTGGCGCTGA12300.17064443941914298No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACC11790.16356893827249558No Hit
GTTTGTATGTGTGTCAGTGCCGACTGAGCGTGTTGGCATGTTTGGCGTGT11410.15829699624166027No Hit
AGCGAAAGCAGGGAAGGGGGTTCGAATGCTGGGTGACTTCAATACCTGTG10920.15149896572821472No Hit
GTTGAATAATGCAGTTGGGGGAGTGTTCGTGTAGATTCTGCTTCTTGTTG10890.15108275977841196No Hit
CACAGAGACACACTGAAACGTCAAGACACATCACGTCAACATCCACGGGA10740.149001730029398No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG10730.1488629947127971No Hit
ATTTAGAGAAACTAGACGAAAACATGAAAACAATGAGTGTAACAGTATGC10510.14581081774757665No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT10410.14442346458156735No Hit
AAGCAGGTGTGAGTGAGGCGCTAGTCATGCGTTGAGATGCCTATAAGCTA10180.14123255229974596No Hit
GGGAAGGGGGTTCGAATGCTGGGTGACTTCAATACCTGTGCCAGGCCACA9850.13665428685191533No Hit
GCTTTTTATTATATGAACAGCTTGATAAACATGGTAGCCAAAAAAAAGTT9620.13346337457009394No Hit
GGTGTGAGTGAGGCGCTAGTCATGCGTTGAGATGCCTATAAGCTAAACGC9550.13249222735388744No Hit
GGTCTGGGGGGGGAAGTCCCATCATATTGCGAGGGCCTCTGGATGCAGTG9500.1317985507708828No Hit
TTCTAAGGGAAAAAAACAAGACCACAGACCAAGGATACAACTCACAGTGT9300.1290238444388642No Hit
GTATAATTGATGACTGCTAGATTGCCCTGTTGAATAATGCAGTTGGGGGA9190.12749775595625398No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT9150.12694281468985028No Hit
CTTATGACACCTGACATGAACTGAAACGGAGACATCCTGCCCCCAAATGT9130.12666534405664842No Hit
CATATATGCTCTTCTTTTCAGGAAGTTCGTTAAAAGGCTTTTTATTATAT9100.12624913810684563No Hit
GATAGGAGCAGTATCTTTTCTCAGTGCACACAGAGACACACTGAAACGTC8630.11972857822660195No Hit
GTATTGGTATTTTCTCTGTGAATTTGCATGTTAGGATGTTCCTACCTTCA8600.11931237227679917No Hit
GGCTTTTTATTATATGAACAGCTTGATAAACATGGTAGCCAAAAAAAAGT8550.1186186956937945No Hit
ACATAAAATACACGCCAAACATGCCAACACGCTCAGTCGGCACTGACACA8480.117647548477588No Hit
AATCTGGACGGCCGGTTCACTGGTGAACAAAAGAAAACGACTGTGCATTT8380.11626019531157872No Hit
GTTTTAACACAGTTTTGAGTTGGGTGGAGAGGTTGATCATCAGCGCCAAG8200.11376295961276199No Hit
GTTCCTGCTCGAGTGTCGAGTGCTTGTTTCCTGTGCCGGATGTCCTTGTT8100.11237560644675268No Hit
TACCAATACTGACGCAATGTATGCAATATGAGGTACATGAAAAAACACAC8030.11140445923054619No Hit
ATTTTATGTTGGTTTGCATTTTCCCGTGGATGTTGACGTGATGTGTCTTG7950.11029457669773876No Hit
GCAGTAGACTGACCATAAATACCACATATACTATTTAGAGAAACTAGACG7940.11015584138113782No Hit
TTCATATACCGGCGATTTTATTCAAGGTTGAAACCGGAAATGCACAGTCG7830.10862975289852761No Hit
ATCTAGCAGTCATCAATTATACATAGATGAAAGTGGAAGTAGGACGTTAA7780.10793607631552295No Hit
GGTAGGAACATCCTAACATGCAAATTCACAGAGAAAATACCAATACTGAC7740.10738113504911923No Hit
TCCTTGGTCTGTGGTCTTGTTTTTTTCCCTTAGAAAGCTCAAAATGATGC7710.10696492909931644No Hit
ATACTATTTAGAGAAACTAGACGAAAACATGAAAACAATGAGTGTAACAG7610.10557757593330716No Hit
ATATGAGGTACATGAAAAAACACACAGATTAAAGCATACTGTTACACTCA7470.10363528150089416No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA7360.10210919301828392No Hit
AAACAAGGCAGGGATTGAAGTGTCATCGGGGCTTTAATTAGTTCCATTAA7350.10197045770168299No Hit
GTATGCAATATGAGGTACATGAAAAAACACACAGATTAAAGCATACTGTT7280.10099931048547649No Hit
TCCTACTTCCACTTTCATCTATGTATAATTGATGACTGCTAGATTGCCCT7280.10099931048547649No Hit
GGTGAGAGGTGTGAAACTAAAGCTGTGAGTAAGCAGAAACATTTGGGGGC7240.10044436921907278No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTTG100.007079618145.074191
AGCGAAA17000.0118.1927951
GCGAAAG19550.0102.74072
CGAAAGC19900.0101.298073
GCAGCTA259.387302E-486.9962168
GAAAGCA27500.074.8746644
CAGGGGC4200.072.496849
ATTAGTA851.6916601E-1068.270211
GCAGGAG754.8548827E-967.663728
CAGGGCC6600.064.8077859
GCAGGGG10000.063.7972268
AAAGCAG35150.063.5293775
GCAGGGC7800.061.3434838
AGCAAAA2900.060.0307041
AGTGATT502.0102989E-457.9974758
CAGGGGG5700.055.9624759
TAGGTGG651.093169E-555.7706764
ACAAGGC2750.055.3727463
AGCAGGT8500.054.5858577
TTAAACG400.006046265454.383952
Produced by FastQC (version 0.11.5)