FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_POSITIVE_FLUB_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_POSITIVE_FLUB_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3339173
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT94440.2828245197238957No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC76250.2283499537160848No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT48620.14560491474984974No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA44860.13434464162234183No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA44330.13275742227192183No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT43090.12904392794263728No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA43060.12895408533789654No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACCA40330.12077840830648787No Hit
GTATAAACACTGGGACAATTGACAAACCACCGGAAGAAATAACTTCTGGA39260.11757402207073428No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA37810.11323162950826447No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT36320.10876944680614031No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT35530.10640359154796711No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGCAAACAAG34300.10272004475359617No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT34030.10191146131092937No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC33530.10041408456525013No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA900.0128.928022
AGCACGC13400.0120.70951
GCACGCA13700.0118.576142
CACGCAC14150.0114.8034443
GTAACAA31250.0109.761094
GAAACAC12900.0102.872364
AAACACG14900.089.5506745
ACGTGCA7800.088.328092
TCGGAAT259.3896507E-486.99774145
ACGTGCC1800.084.609022
CGCTCCC353.4388995E-582.854996145
CGCACTT21250.080.194815
GAGCAGA2550.079.6451341
ACGCACT21500.079.2623144
CGTGCCT1850.078.4013
CAACGCA8000.076.1230249
AACACGA14100.075.6023946
TAGTAAC46250.075.109282
TAACAAG46000.075.0388955
CACGTGC11300.074.459641
Produced by FastQC (version 0.11.5)