FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_PLASMID_CONTROL_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_PLASMID_CONTROL_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2433611
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT134080.5509508298573601No Hit
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT96210.3953384497358041No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA70590.2900627914650287No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG68400.2810638183341545No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG61170.25135487964181624No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA59930.2462595706544719No Hit
TCTAATAACAGTTTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATT57660.2369318679115109No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT55140.2265768851307789No Hit
ATTGTATGTTGGTTCTCCAGGGCAACAAGAAGCTCCGCGTTGTATGACCA54900.22559069629451872No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT52980.21770118560443721No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA51650.2122360558034953No Hit
CTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGGAG51510.21166077898234353No Hit
GTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCATTTTGAGTGACTC48530.1994156009321128No Hit
TCATAAGGGTAACAGTTGCTGTGGGCTTTGCTGCGTTCAACAAAAAGGTC48000.19723776725203823No Hit
CATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCA45380.1864718724561978No Hit
CCCTTACCCTGGGTCTAGATCCGATATTCGGGATTACAGTTTGTTGGCTT45100.18532131881389424No Hit
CTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAG43480.17866454416913796No Hit
ACATACAATTGATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAGAA43480.17866454416913796No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC42980.1766099840935959No Hit
ATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCTAC42710.1755005216528032No Hit
TAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCTAATT42200.17340487037575028No Hit
GTCCAATAGATGCTTATTCTGCTGGGGATATCCCTTACCCTGGGTCTAGA40770.16752882855969997No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGGATGCGA39170.16095423631796538No Hit
TCTCAAAAGCACTCAAGCAGCAATCAACCAAATCAATGGGAAGCTGAATA38370.15766694019709807No Hit
CTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCT37850.1555301977185343No Hit
CTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGC37420.15376327605356813No Hit
GTCCGTACCCGGGTGGTGAACTCCCCAAATGTACAATTTGTCAAAATTTT37080.15236617520219953No Hit
TCTCTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCA36900.15162653357500439No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC36350.1493665174919081No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG35840.1472708662148552No Hit
ACATTCTATGTCTGGTTTTCGCTCAAAAACTTCCCGGAAATGACAACAGC35580.14620249497557333No Hit
GATGAGGACTGTCGCATATTCCACCTGTTGAGGAACTCTGAACCAGCTCA35520.14595594776650828No Hit
CTATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCT35000.14381920528794453No Hit
ATGCAGGCATTGTCACATTTGTGGTATATTTTGAAACAACCATTGCCCAT34920.14349047567585782No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT34790.14295629005621688No Hit
GGTACGGACAATGACCAAATCAGCCTATATGCTCAAGCATCAGGAAGAAT34120.1402031795549905No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT32890.13514896176915703No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC32390.13309440169361497No Hit
TGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATC32360.13297112808908243No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT32250.1325191248724632No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT32250.1325191248724632No Hit
ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG32020.13157402723771383No Hit
ATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGTTGAGC31910.13112202402109457No Hit
ATCTATTAGTGTGCAGTTTTCTCCATCAAGGATCTGATGAGGACTGTCGC31610.12988928797576935No Hit
ACCCTATGCAGGCATTGTCACATTTGTGGTATATTTTGAAACAACCATTG31290.12857436952742243No Hit
GTTTTACTATTGTCCAATAGATGCTTATTCTGCTGGGGATATCCCTTACC31000.127382724683608No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT30210.12413651976425155No Hit
TTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATTCAAATACCCAGC30200.12409542856274072No Hit
ACTAAAGAAACTGTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCAT30000.1232736045325239No Hit
AACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAG29620.12171213887511192No Hit
CCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTCTTTCAAACAG29520.12130122686000351No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCTTT29330.12052049403129751No Hit
TATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAAC28900.11875357236633136No Hit
GATCTAGACCCAGGGTAAGGGATATCCCCAGCAGAATAAGCATCTATTGG28880.11867138996330966No Hit
GGGTAAGGGATATCCCCAGCAGAATAAGCATCTATTGGACAATAGTAAAA28870.11863029876179883No Hit
ATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCTTT28860.11858920756028798No Hit
GTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGTTGA28550.11731538031345191No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG28480.11702774190287601No Hit
TTTGGGGAGTTCACCACCCGGGTACGGACAATGACCAAATCAGCCTATAT28350.11649355628323507No Hit
CTATTAACCATGAAGACTATCTTTGCTTTGAGCTACATTCTATGTCTGGT28300.11628810027568086No Hit
TACTAATGCTACTGAGCTGGTTCAGAGTTCCTCAACAGGTGGAATATGCG28170.11575391465603994No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT28140.11563064105150742No Hit
ATGTGATCCTGTTTACATTTTGAAATGGTTTGTCATTGGGAATGCTTCCA28050.11526082023790983No Hit
ATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGC27790.11419244899862797No Hit
CCCTTATGATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGCCTCAT27630.1135349897744545No Hit
GGTATATCTTTCGCTCCGAGTCGGCATTTTGGGCCGCCGGGTTATTAGTA27470.11287753055028103No Hit
GCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAG27310.11222007132610759No Hit
ACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATA27240.1119324329155317No Hit
ATTAGATTCCCTGTGCTGTTAATCAAAAGTATGTCTCCCGGTTTTACTAT27070.1112338824898474No Hit
CACTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGG26930.1106586056686956No Hit
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCG26900.11053533206416309No Hit
CTACCAAAAGAAGCCAACAAACTGTAATCCCGAATATCGGATCTAGACCC26560.10913823121279449No Hit
GTCCTATTCCCTCAGAATTTTGATGCCTGAAACCGTACCAACCATCCACC26490.10885059280221861No Hit
TTCTATGTCTGGTTTTCGCTCAAAAACTTCCCGGAAATGACAACAGCACG26140.10741240074933914No Hit
GTCATTGGGAATGCTTCCATTTGGAGTGATGCATTCAGAATTGCATTTGC26100.10724803594329578No Hit
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGA25890.10638512071156811No Hit
GTCCTGAATTCTCCCTTCTACTTCTGAGAATTCTTTTTCAATCTGATGGA25890.10638512071156811No Hit
GAATAGGACAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCAACCAA25880.10634402951005728No Hit
ACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGA25820.10609748230099222No Hit
GGATCACATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAA25820.10609748230099222No Hit
TTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCC25340.10412510462847184No Hit
ATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT25180.10346764540429837No Hit
TGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGTTG25130.10326218939674418No Hit
GTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATA24990.1026869125755924No Hit
TCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTG24780.10182399734386474No Hit
GGATATGGGCAATGGTTGTTTCAAAATATACCACAAATGTGACAATGCCT24700.101495267731778No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT24600.1010843557166696No Hit
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATT24470.10055017009702866No Hit
GAACGTGACTATGCCAAACAATGAAAATTTTGACAAATTGTACATTTGGG24390.10022144048494191No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA3150.075.973152
TTCGGAC3350.071.431563
CGGACAT3550.067.405875
TCGGACA3600.066.469674
ATACAGG451.193193E-464.4554446
GGACATC3750.063.8108866
CTTTCGG3850.062.182851
AGAGTGG2550.059.7160725
GACATCT4250.056.2921527
CGTCCTT651.0943391E-555.76729145
CCAGAGT2800.054.38543
TTAAGAT400.006047839854.384284
TAGAGGG553.2204748E-452.725437145
CTCGTAA553.2204748E-452.725437145
AGGAGAC450.00964211148.33165145
TGCGAGC450.00964211148.33165145
GTCCAGA3250.046.8763051
CCGCTAG2000.043.5118942
GAGTGGC3500.043.5074236
AGTGGCC3550.042.885837
Produced by FastQC (version 0.11.5)