FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_NEGATIVE_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_NEGATIVE_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9588
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAG630.6570713391739674No Hit
GCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGC350.36503963287442637No Hit
CTCATGACTTGAGCCTCATGCCTCAATGAATTACGCTGGCATCGTATCCC160.1668752607425949No Hit
GTAGTAACAAGAGCCGGGTTATTAGTAGTAACAAGAGCCGGGTTATTAGT150.15644555694618273No Hit
GTAGTAACAAGAGCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAG150.15644555694618273No Hit
TCCCCACACCCACCAAAGTCATGAGGCGATCATCAAGCGCCTCAAACGAG130.13558614935335836No Hit
GTAGTAACAAGAGGATTGGGGGGAGCAGAAGCAGAGGATTGGGGGGAGCA130.13558614935335836No Hit
TCCAAGATCACATTGATCACTGCCTTGAGGACACAGTTTCGGCGCTGGGC120.1251564455569462No Hit
AGTAGAAACAACGCACTTGGGGGGAGCAGAAGCACGCACTTGGGGGGAGC120.1251564455569462No Hit
GCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGC110.114726741760534No Hit
CTAATAACCCGGGCTCTTGTTACTACTAATAACCCGGCTCTTGTTACTAC100.10429703796412182No Hit
GAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGA100.10429703796412182No Hit
GTAGTGGGCGGTGCGATGGCGCAGTGAATGCGACAACGCCACTCATTGAG100.10429703796412182No Hit
TTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTT100.10429703796412182No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGCG151.19250224E-4145.05
AGAGCGT100.006982045144.999986
AAGCGGA300.001877360272.54
AGCGGAG350.00345932862.1428575
GCGGGGG400.005869716454.3749969
GAGCGTG400.005869716454.3749967
AGCGTGC400.005869716454.3749968
GCGGAGC450.00935158248.3333366
ACGAGCC803.4626035E-545.3124969
AGTAACA854.9532864E-542.6470573
GTAGTAA854.9532864E-542.6470571
TAGTAAC906.939881E-540.277782
AAGAGCC750.001410953938.6666649
GGATTGG959.544564E-538.1578941
GTAACAA959.544564E-538.1578944
ATTGGGG1001.2910177E-436.253
GATTGGG1001.2910177E-436.252
AACACGA1301.4905145E-533.461546
GAAACAC1758.4333806E-833.1428574
AAACACG1801.0802978E-732.2222255
Produced by FastQC (version 0.11.5)