FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_CM554145_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_CM554145_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921027
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2800.090.665191
AGCGAAA6150.070.763081
GCGAAAG6350.068.519432
CGAAAGC6700.064.9400563
GAGCAAA16800.063.0338971
CAAAAGC21350.048.910364
TATCGTG3850.047.0885732
GCAAAAG23700.044.06063
TCGTGTA4150.043.6845784
CTACGCC2400.042.289753145
ATATCGT4300.042.1698531
ATCGTGT4350.041.676093
CGTGTAG4350.041.676095
AGCAAAA25700.040.067432
AAAAGCA27800.038.3450055
AAAGCAG34550.037.1502236
AAGCAGG37450.034.2641337
TACGTTC1102.4366488E-432.9530537
GACCCGT900.003624067232.229516
TAATACG1153.1577155E-431.528874
Produced by FastQC (version 0.11.5)