FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_CM554007_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_CM554007_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263414
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA46030.20336535870150138No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT33860.1495970246715802No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA31660.13987719436214496No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC30910.1365636158475648No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT30900.13651943480070372No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG30690.13559163281662126No Hit
TCCTTATTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT26510.11712395522869436No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG26390.11659378266636153No Hit
ATTATATTCAGTATGGAGAGAATAAAAGAACTACGGAATCTGATGTCGCA26290.11615197219775084No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT24720.10921554784056298No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG23210.10254420976454152No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG100.007092157144.99516145
TCACGCA1300.078.0915762
ACGCAGT1300.072.50884
CACGCAG1450.070.011583
GAGCGAA11100.066.002721
TACGACG554.0592713E-665.923
GCAAAAG56900.064.101123
CGAAAGC14550.062.295533
ATTACGA606.7874407E-660.4480361
TTACGAC606.8007957E-660.4279982
AAGCAGG75100.058.5965777
GTATAAT502.0056369E-458.030111
CAAAAGC64550.057.6251144
ACGACGG651.0933027E-555.7759974
AGCAAAA66800.055.2536242
CGTGTAG5650.055.1836855
TATCGTG5700.054.7032432
ACGTCAA400.00605266854.373188145
GAGCAAA70300.053.0360571
AGCGAAA17150.052.447041
Produced by FastQC (version 0.11.5)