FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_CM553981_2_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_CM553981_2_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2069956
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA50380.2433868159516434No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC38370.1853662589929448No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC37210.1797622751401479No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC34930.1687475482570644No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA28760.13894015138486035No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT28050.13551012678530364No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC28050.13551012678530364No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC25570.12352919578966896No Hit
GAACCAGTTAAGAGGCTGTTTTTGATCTGGGACAGAGAGAGGGTGATGAC24550.11860155481565793No Hit
CACTCTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAG23850.11521984042172878No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA23100.11159657499966182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA42350.0103.9639741
GAGCGAA10150.0102.192161
AGCAAAA56600.077.386132
CAAAAGC62800.069.3946464
GCAAAAG65500.066.980163
AAAGCAG82500.065.920196
AGCGAAA16300.065.859991
TACGACG2050.063.6723333
AAAAGCA70150.062.7439965
CGACGGG2100.062.1533245
GCGAAAG17650.061.218712
CGAAAGC18700.058.1676863
ATTACGA2250.058.0279921
ACGACGG2250.058.009774
CGCAGTC3800.057.2464875
TCACGCA4050.053.716642
ATCGTGT17850.052.0000653
TATCGTG18650.051.326042
CGTGTAG18400.050.443285
TCGTGTA18450.050.306584
Produced by FastQC (version 0.11.5)