FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_CM553980_2_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_CM553980_2_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2503845
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA57740.23060532900399186No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT40220.16063294652823956No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA39050.15596013331496159No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG39020.1558403175915442No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT37250.14877118990991853No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC35870.14325966663271886No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG33270.13287563726987894No Hit
TCCTTATTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT32810.1310384628441457No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT30230.12073431063025068No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA30110.12025504773658113No Hit
ATTATATTCAGTATGGAGAGAATAAAAGAACTACGGAATCTGATGTCGCA29670.11849775045979284No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT28110.11226733284208885No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC27730.11074966701213533No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC27680.11054997413977304No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG27200.1086329225650949No Hit
TTCTTAGACAGAACCCGACAGAAGAACAAGCTGTGGATATATGCAAGGCT26130.10435949509654152No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGG100.0070789987145.085561
GGCTCGG100.0070789987145.085561
CAGCCGG100.0070925406144.99284145
TACTAAT2050.081.337457
GCAGGGG7100.080.665049
TAAACTA5350.073.192965
GCAGGGT11700.073.116059
ATTAGTA1100.072.542781
TTTAAAC5400.072.518153
CGGAGAT300.001934468672.515255
ACCTTCA300.001936462172.49642145
CGCGTAT651.3993849E-766.91978145
AGGGTGT6800.060.7848436
CGAAAGC14000.059.568483
GCAAAAG58900.053.926913
AAGCAGG70550.053.845687
AGACGCC1353.6379788E-1253.701057145
TTATATT9350.052.742582
TATATTC9900.049.8104483
GCTTATA4550.049.4099432
Produced by FastQC (version 0.11.5)