FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_CM553698_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_CM553698_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456276
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA25800.1771642188705987No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC23770.16322455358736943No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC18680.1282723879264645No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC18270.12545698754906348No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC15550.10677920943557402No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6450.088.863531
TACGACG1200.072.515773
CGACGGG1200.072.510795
AGCGAAA11500.068.136861
ACGACGG1400.067.331444
ATTACGA1300.066.972131
CGAAAGC12050.065.595183
CAAAAGC37400.065.337264
CGCTACC451.1941766E-464.44296145
GCAAAAG39150.062.9766543
CTCGAGG350.003556492362.1884041
GCGAAAG12900.060.7108732
GAGCAAA42650.058.1786041
AGCAAAA45900.053.715382
CGTGTAG9750.052.8027235
TATCGTG9800.052.536932
AAAGCAG61500.049.4016576
ATATCGT10600.048.5969121
AAGCAGG66400.046.184937
TCACGCA3000.045.9266472
Produced by FastQC (version 0.11.5)