FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_CM553445_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_CM553445_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1933996
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA14400.071.010991
CGAAAGC14800.069.570983
GCGAAAG16150.063.306472
GAGCGAA8350.060.7967641
GCAAAAG39750.057.461233
GAGCAAA40950.056.494441
AGCAAAA43100.052.6585122
CAAAAGC50000.046.6956184
ACGACGG1851.8189894E-1243.1132974
TCACGCA2450.041.434632
TACGACG1755.2750693E-1141.4346283
CGACGGG1807.2759576E-1140.2826275
GGTCCGA907.479729E-540.2826276
AATCGAC907.486571E-540.27638145
TTACGAC1859.640644E-1139.194922
ATTACGA1901.2914825E-1038.16941
AAGCAGG74550.037.6345067
AAAGCAG74250.037.597126
TATCGTG9600.035.499982
TAGAAAC16800.035.392082
Produced by FastQC (version 0.11.5)