FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_CM552690_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_CM552690_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908070
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA30700.1608955646281321No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC29220.15313903577960974No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC25080.13144171859522974No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC24000.12578154889495669No Hit
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC22730.11912560859926524No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC20930.10969199243214348No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC19580.10261678030680216No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA19550.10245955337068346No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA19190.10057283013725912No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8550.092.4912341
GAGCAAA36600.080.083031
TCACGCA3500.066.314462
AGCAAAA45000.064.955942
AGCGAAA14000.064.258981
GCGAAAG15300.058.7836572
CGAAAGC15300.058.7790343
GCAAAAG52050.056.710813
CAAAAGC52150.056.3194924
GCCGAAT400.00604323154.3943063
CGCAGTC4700.052.4613045
GTCGGCT7550.049.931879
CGACGGG1900.049.6189775
AAAGCAG75950.048.9832536
CAGTCGG7850.048.0236477
TACGACG2000.047.1417353
CGTGTAG13600.044.791795
ACGCAGT5250.044.202694
GCAGTCG8650.043.595866
TATCGTG14450.043.1675072
Produced by FastQC (version 0.11.5)