FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_CM552654_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_CM552654_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049928
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC40660.13331462250912154No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC34320.11252724654483647No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA31770.10416639343617293No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA12750.068.843221
AGCGAAA20250.063.048341
CGACGGG2450.062.1538855
CGAAAGC21100.061.517673
ACGACGG2500.060.9108054
TACGACG2550.059.7184333
TCACGCA3750.058.0162
GAGCAAA65750.057.5918121
TTACGAC2650.057.4686782
GCGAAAG22950.056.562442
ATTACGA2750.055.3952561
AGCAAAA70100.053.174442
GCAAAAG70600.052.588963
CAAAAGC77850.048.4350554
GTCGGCT7850.047.1002279
GCAGTCG8300.044.55616
ACGCAGT5250.042.817124
CACGCAG5600.041.4372833
GACGTAA700.001056059841.435926
CATACCG1755.2750693E-1141.427099
Produced by FastQC (version 0.11.5)