FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_CM552549_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_CM552549_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173201
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA147240.6775259168388014No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC130960.6026133799864808No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG124540.5730717039058973No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC88070.4052547371366017No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG86130.39632781321193944No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT61920.28492532444076735No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT61370.2823944954930538No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC54000.24848138759369243No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCAGTCTCGCACT52560.24185521725786063No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT52330.24079687060699861No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG51970.2391403280230407No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG51400.2365174689317739No Hit
GAATAAAAGAACTACGGAATCTGATGTCGCAGTCTCGCACTCGCGAGATA49030.22561189692071743No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA45930.21134722466996841No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC45890.21116316438286195No Hit
TCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAATGTGCTAATC43540.20034962251535868No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG42570.19588616055302754No Hit
GCATTAAGCATCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAA42200.19418360289729297No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC41810.19238901509800518No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA39340.1810232923691826No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA38320.1763297550479684No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT38150.17554749882776605No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT38070.17517937825355318No Hit
GAGTAACCTTGCAAAAGGGGAAAAGGCTAATGTGCTAATCGGGCAAGGAG36850.1695655394968068No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT35360.16270929380209193No Hit
TTCTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC35080.16142087179234688No Hit
TCTTAATTATGGCCATATGGTCCACTGTGGTTTTTGTCAGTATCTCGCGA34990.16100673614635738No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC34550.15898207298818656No Hit
CTAGTATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC33840.15571500289204726No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA33790.1554849275331642No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC33510.15419650552341913No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT33090.15226387250880152No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC32280.14853665169489613No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC31800.14632792824961888No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG30730.14140431556952165No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA29950.13781513997094608No Hit
GAAATATCCAATCACTGCTGACAAAAGATAGGTAAGGAAGACAGAAGATA29780.1370328837507437No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG28720.13215528614242308No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA28070.12916430647694344No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA26990.12419467872506962No Hit
GGATATTTCATTGCCATCATCCATTTCATCCTAAGTGACGGGTTCTTTTC26610.12244610599755844No Hit
GCTTAATGCTGGTCCGTATCTTCTGTCTTCCTTACCTATCTTTTGTCAGC26360.1212957292031432No Hit
GTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAATGCATGTGTC26020.11973121676273847No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG25590.11775256867634426No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC25450.11710835767147171No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT25200.11595798087705646No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT25110.11554384523106698No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT24610.11324309164223649No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA24530.11287497106802363No Hit
CAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCG24330.11195466963249143No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA24090.11085030790985279No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT23580.1085035392492457No Hit
GGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATG23570.1084575241774691No Hit
GTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAATGGA23340.10739917752660706No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATC23330.10735316245483045No Hit
AAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC22950.10560458972731929No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT22890.10532849929665963No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTACC22760.1047303033635637No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCCTTACCTATC22490.10348789642559524No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG22190.10210744427229694No Hit
ATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCAGTC22130.1018313538416373No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG22060.10150924833920102No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGC203.867396E-4108.744896145
CAAAAGC77550.075.178184
GAGCGAA16450.073.639031
GTACTAC300.001934289672.516623
GCAGGGG5900.072.49669
GCAGGTC36000.068.670389
GCAGGGT15750.067.663499
CGTGTAG451.1930906E-464.4562455
AAGCAGG108100.064.113557
CGAAAGC22450.062.98773
AGCAAAA93150.062.824382
GTGACGA350.003559932662.1742671
TACCGTC350.003567730562.13994145
AGCAGGG24150.061.839758
GAGCAAA97700.060.6575621
AGTAGAA20200.059.609321
GCGAAAG24350.058.3706632
TATCGTG502.0084952E-458.0132942
GCAGGTA34650.056.9093069
AGCAGGT80000.055.8223768
Produced by FastQC (version 0.11.5)