FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_CM552492_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_CM552492_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538099
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT32590.12840318679452614No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC32360.12749699676805357No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC28960.1141011442028069No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT28080.11063398236239011No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA27520.10842760664576126No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA27090.10673342529192123No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG25450.10027189640750814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC18250.070.728453
GCAAAAG56250.065.619413
GAGCGAA12850.062.661731
AGCGAAA20650.062.1778561
GCGAAAG20900.061.7629242
AGCAAAA65350.057.260912
GAGCAAA67250.057.169731
CAAAAGC66550.056.6587034
CGACGGG1450.055.009295
ACGACGG1700.051.1851164
ACGCAGT2900.050.0084464
TACGACG1600.049.8551143
TTACGAC1650.048.3462642
ATGCGGC604.9476966E-448.331029
TCACGCA2900.047.5127032
AAAAGCA93700.040.860695
GTCGGCT5800.039.9980859
ATTACGA2005.456968E-1239.8974571
TATCGTG11100.039.8529972
AAGCAGG113200.039.7706267
Produced by FastQC (version 0.11.5)