FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_CM551963_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_CM551963_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199580
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA33030.1501650315060148No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC27800.1263877649369425No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC24210.11006646723465388No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC24050.10933905563789452No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA10250.077.129011
TACGACG2150.067.4463653
GAGCAAA53950.064.93381
CGACGGG2250.064.4487465
ACGACGG2250.064.4487464
CGAAAGC15500.064.084923
TTACGAC2300.063.0519872
ATTACGA2200.062.6391831
GCAAAAG55750.062.5557483
TCACGCA2700.061.7675932
AGCGAAA16350.060.330411
CAAAAGC58800.059.927474
AGCAAAA58250.059.626082
GCGAAAG16900.058.780132
AAACGTC553.2205973E-452.7248145
AAAGCAG89750.047.582576
CGTGTAG12650.044.7065435
AAGCAGG96700.044.6824957
TATCGTG12950.043.6738472
AAAAGCA84200.043.1412435
Produced by FastQC (version 0.11.5)