FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_CM551457_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_CM551457_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28329
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGGAGAGCGCTTGCATGGCATGCAAGAGGTCAGCGGTTCGATCC1390.49066327791309255No Hit
AGCAGGGAGAGCGCTTGCATGGCATGCAAGAGGTCAGCGGTTCGATCCCG1040.3671149705249038No Hit
TTTCAGATCCGTCTGACTTTCGCTATTACTTCCGGTCAACAGCGAAGGCC1020.36005506724557873No Hit
TCGTAGAAGTGTCGCGAGGCTTGCAGGAACGTAACCGACGTCCTGCAGGT960.3388753574076035No Hit
CCCCTGTACTCACCGTGAAAGGGTGATGTCCTAGGCCTCTAGACGAAGGG950.335345405767941No Hit
GGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGG830.29298598609199056No Hit
AGCGAAAGCAGGGAGAGCGCTTGCATGGCATGCAAGAGGTCAGCGGTTCG830.29298598609199056No Hit
CAGGAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGA810.28592608281266546No Hit
CTTCTACGAAACGCCGCTGAATCTGGCGTCCCCTAGGGGATTCGAACCCC810.28592608281266546No Hit
CTTCTACGAAACGCCGCTAAATCTGGCGTCCCCTAGGGGATTCGAACCCC800.28239613117300294No Hit
TATTACTACCGCTGCTTCTCAAATCAATCCAGCTTGTTCTGTTGGTGGAG760.2682763246143528No Hit
GTCTTCGGGCGTACCGACGCGTCCGAGCGGATGAAACGAATCGAGCGCTT760.2682763246143528No Hit
AAGCAGGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGAT740.2612164213350277No Hit
TCTCAAATCAATCCAGCTTGTTCTGTTGGTGGAGGTAAGCGGGATCGAAC730.2576864696953652No Hit
AAGCAGGAGAGGGGGAGCAGGAGAAGGAGAAGGAGAAGGGGAAGGAGAAG710.2506265664160401No Hit
CCCCTAGGGGATTCGAACCCCTGTACTCACCGTGAAAGGGTGATGTCCTA690.24356666313671504No Hit
GTAGTAATAAATGCTTCACAAAATGATTCAAAGCAGTTAGAATTTCGGCC650.2294468565780649No Hit
GTCTAGAGGCCTAGGACATCACCCTTTCACGGTGAGTACAGGGGTTCGAA640.22591690493840236No Hit
AGCGAAAGCAGGAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGAC630.2223869532987398No Hit
GCCTAGGACATCACCCTTTCACGGTGAGTACAGGGGTTCGAATCCCCTAG610.21532705001941474No Hit
ATCTAAGAAGGGCATCAAGTGATGTTCTAGTGAAGCGTTTAGGAGGCCGT610.21532705001941474No Hit
AGCGAAAGCAGGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCG590.20826714674008964No Hit
GCATGCAAGAGGTCAGCGGTTCGATCCCGCTTACCTCCACCAACAGAACA570.2012072434607646No Hit
ATTCAAAGCAGTTAGAATTTCGGCCTTCGCTGTTGACCGGAAGTAATAGC560.19767729182110205No Hit
CTGTACGATCGCCGGCGAGACAGCGTTCACCCGGATACCCGCATCGGCGA560.19767729182110205No Hit
CTTAGATACCGCCTTTCCCATCGAGATCAAACACTCTCCTTCTCTCATCC550.1941473401814395No Hit
GTAATAAATGCTTCACAAAATGATTCAAAGCAGTTAGAATTTCGGCCTTC550.1941473401814395No Hit
CTCCCAGGGAGGGAGATTAGCATGATGGAGAGACATTGAGAAAATGAAAT540.19061738854177698No Hit
CCTGTACTCACCGTGAAAGGGTGATGTCCTAGGCCTCTAGACGAAGGGGA530.18708743690211443No Hit
CTTTAATACAAAAGCCCGCTCAAAAACGTCTTGAACGGGCTTCGTTCTGG530.18708743690211443No Hit
CCCTAGGGGATTCGAACCCCTGTACTCACCGTGAAAGGGTGATGTCCTAG520.1835574852624519No Hit
GTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAATCTGGCGT520.1835574852624519No Hit
ATCGTACAGACGTCGATTTATGAAGGCTTCATGGCGAAGGAAGACATTGC510.18002753362278937No Hit
AGCGAAAGCAGGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTT490.1729676303434643No Hit
GATTTGAGAAGCAGCGGTAGTAATAAATGCTTCACAAAATGATTCAAAGC480.16943767870380175No Hit
GGCTTCGTTCTGGCGGAGCGGACGGGACTCGAACCCGCGACCCCCGGCGT470.16590772706413923No Hit
AGCGAAAGCAGGAGAGGGGGAGCAGGAGAAGGAGAAGGAGAAGGGGAAGG470.16590772706413923No Hit
GTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCC470.16590772706413923No Hit
GATCTGAAAAGATCATGTCCCCTTCGTCTAGAGGCCTAGGACATCACCCT470.16590772706413923No Hit
ATACAAAAGCCCGCTCAAAAACGTCTTGAACGGGCTTCGTTCTGGCGGAG460.16237777542447668No Hit
AGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGAGCGGA460.16237777542447668No Hit
GAGTAAAACACTCCAGTGTTCTCTCTCCCACGGCCTCCTAAACGCTTCAC460.16237777542447668No Hit
ATTCTAACCGACTGAACTACCGCTCCAACTTTCTGCACCGTTACCTGCAG460.16237777542447668No Hit
AGCGAAAGCAGGACATGGAGAGCCAGAGAGATATGAGAGGAAGAGGATGG450.15884782378481416No Hit
ACAAAAGCCCGCTCAAAAACGTCTTGAACGGGCTTCGTTCTGGCGGAGCG450.15884782378481416No Hit
GTATTAAAGATGTAAGCAGTACGTTGTCCCCTTCGTCTAGAGGCCTAGGA450.15884782378481416No Hit
GTCCTAGGCCTCTAGACGAAGGGGACATGATCTTTTCAGATCCGTCTGAC440.1553178721451516No Hit
AAGGAAGACATTGCAGGTGCGATGAAAGCGCTCGATTCGTTTCATCCGCT440.1553178721451516No Hit
AAATGATTCAAAGCAGTTAGAATTTCGGCCTTCGCTGTTGACCGGAAGTA430.1517879205054891No Hit
GCTTACATCTTTAATACAAAAGCCCGCTCAAAAACGTCTTGAACGGGCTT430.1517879205054891No Hit
GTCTTCCTTCGCCATGAAGCCTTCATAAATCGACGTCTGTACGATCGCCG430.1517879205054891No Hit
GGCCTAGGACATCACCCTTTCACGGTGAGTACAGGGGTTCGAATCCCCTA430.1517879205054891No Hit
GGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGC420.14825796886582654No Hit
GGACATGGAGAGCCAGAGAGATATGAGAGGAAGAGGATGGATGGATGGAT410.14472801722616402No Hit
TTGCAGGTGCGATGAAAGCGCTCGATTCGTTTCATCCGCTCGGACGCGTC410.14472801722616402No Hit
CAGGGAGAGCGCTTGCATGGCATGCAAGAGGTCAGCGGTTCGATCCCGCT400.14119806558650147No Hit
GTTTAGGAGGCCGTGGGAGAGAGAACACTGGAGTGTTTTACTCTCTTCCT400.14119806558650147No Hit
CTCAAAAACGTCTTGAACGGGCTTCGTTCTGGCGGAGCGGACGGGACTCG400.14119806558650147No Hit
CTTGCATGGCATGCAAGAGGTCAGCGGTTCGATCCCGCTTACCTCCACCA400.14119806558650147No Hit
ATTTATTACTACCGCTGCTTCTCAAATCAATCCAGCTTGTTCTGTTGGTG390.13766811394683892No Hit
AGTAATAAATGCTTCACAAAATGATTCAAAGCAGTTAGAATTTCGGCCTT390.13766811394683892No Hit
CTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGGTATCCGGGTGAA390.13766811394683892No Hit
GTATCTAAGAAGGGCATCAAGTGATGTTCTAGTGAAGCGTTTAGGAGGCC390.13766811394683892No Hit
TTTATTACTACCGCTGCTTCTCAAATCAATCCAGCTTGTTCTGTTGGTGG390.13766811394683892No Hit
GAAGTAATAGCGAAAGTCAGACGGATCTGAAAAGATCATGTCCCCTTCGT390.13766811394683892No Hit
AGCGAAAGCAGGGCGAGAGGAGAGGAGAGGAGAGGAGAGGAGAGGAGAGG390.13766811394683892No Hit
AGCGAAAGCAGGGGCCCTCCACTGAGTGAGTGCATTAAACAAAGCAGACA380.1341381623071764No Hit
AATAAATGCTTCACAAAATGATTCAAAGCAGTTAGAATTTCGGCCTTCGC380.1341381623071764No Hit
GAGTACAGGGGTTCGAATCCCCTAGGGGACGCCAGATTTAGCGGCGTTTC380.1341381623071764No Hit
GTACAGGGGTTCGAATCCCCTAGGGGACGCCAGATTCAGCGGCGTTTCGT370.13060821066751385No Hit
GTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGGT370.13060821066751385No Hit
GAAAAGATCATGTCCCCTTCGTCTAGAGGCCTAGGACATCACCCTTTCAC360.1270782590278513No Hit
GTACAGGGGTTCGAATCCCCTAGGGGACGCCAGATTTAGCGGCGTTTCGT360.1270782590278513No Hit
TTCTTAGATACCGCCTTTCCCATCGAGATCAAACACTCTCCTTCTCTCAT360.1270782590278513No Hit
GAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGAGCG350.1235483073881888No Hit
GACATGGAGAGCCAGAGAGATATGAGAGGAAGAGGATGGATGGATGGATA340.12001835574852625No Hit
GAAAAGAGAGAGGAAGAGAGTAAAACACTCCAGTGTTCTCTCTCCCACGG340.12001835574852625No Hit
ATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAA340.12001835574852625No Hit
CCCTAGGGGACGCCAGATTTAGCGGCGTTTCGTAGAAGTGTCGCGAGGCT340.12001835574852625No Hit
GGAGAGGGGGAGCAGGAGAAGGAGAAGGAGAAGGGGAAGGAGAAGGAGGA330.1164884041088637No Hit
GTAATAGCGAAAGTCAGACGGATCTGAAAAGATCATGTCCCCTTCGTCTA330.1164884041088637No Hit
CTATTACTTCCGGTCAACAGCGAAGGCCGAAATTCTAACTGCTTTGAATC330.1164884041088637No Hit
GCAGGAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCG330.1164884041088637No Hit
TCTTAGATACCGCCTTTCCCATCGAGATCAAACACTCTCCTTCTCTCATC320.11295845246920118No Hit
CCCCTAGGGGACGCCAGATTTAGCGGCGTTTCGTAGAAGTGTCGCGAGGC320.11295845246920118No Hit
GTTCTGTTGGTGGAGGTAAGCGGGATCGAACCGCTGACCTCTTGCATGCC310.10942850082953863No Hit
GGAGAAGGAGAAGGAGAAGGGGAAGGAGAAGGAGGAGAGTATTTGATAGC310.10942850082953863No Hit
TCACAAAATGATTCAAAGCAGTTAGAATTTCGGCCTTCGCTGTTGACCGG310.10942850082953863No Hit
TTTCTGCACCGTTACCTGCAGGACGTCGGTTACGTTCCTGCAAGCCTCGC310.10942850082953863No Hit
GTTCGATCCCGCTTACCTCCACCAACAGAACAAGCTGGATTGATTTGAGA310.10942850082953863No Hit
GTTAGAATTTCGGCCTTCGCTGTTGACCGGAAGTAATAGCGAAAGTCAGA300.1058985491898761No Hit
TTCCTGCAAGCCTCGCGACACTTCTACGAAACGCCGCTAAATCTGGCGTC300.1058985491898761No Hit
GAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTC300.1058985491898761No Hit
TCCTAGGCCTCTAGACGAAGGGGACATGATCTTTTCAGATCCGTCTGACT300.1058985491898761No Hit
CTATAAGCCAACGCGGTGCTGCCGGCGGTAACGGTTTGTCAGGTGCGCCG300.1058985491898761No Hit
GCATTTATTACTACCGCTGCTTCTCAAATCAATCCAGCTTGTTCTGTTGG300.1058985491898761No Hit
ATTACTTCCGGTCAACAGCGAAGGCCGAAATTCTAACTGCTTTGAATCAT290.10236859755021355No Hit
GTATTCTAACCGACTGAACTACCGCTCCAACTTTCTGCACCGTTACCTGC290.10236859755021355No Hit
GATCTTTTCAGATCCGTCTGACTTTCGCTATTACTTCCGGTCAACAGCGA290.10236859755021355No Hit
CCTGCAAGCCTCGCGACACTTCTACGAAACGCCGCTAAATCTGGCGTCCC290.10236859755021355No Hit
ATTTATGAAGGCTTCATGGCGAAGGAAGACATTGCAGGTGCGATGAAAGC290.10236859755021355No Hit
GTTACCTGCAGGACGTCGGTTACGTTCCTGCAAGCCTCGCGACACTTCTA290.10236859755021355No Hit
GCTTTGAATCATTTTGTGAAGCATTTATTACTACCGCTGCTTCTCAAATC290.10236859755021355No Hit
GAAGAGGATGGATGGATGGATAGATGGATGGATGGATGGATGGCTGGATG290.10236859755021355No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAAC100.007054756145.0145
AGTAGCC100.007054756145.02
GCAGGGT100.007054756145.08
CCATGTG100.007054756145.02
CCCATGT100.007054756145.01
ATGTGCT100.007054756145.04
GAGCAAA100.007054756145.01
CATGTGC100.007054756145.03
CGAAAGC1100.098.863633
GAAAGCA1200.090.6254
AGCAGGC553.9606384E-665.909097
GCGAAAG1750.062.1428572
AGCGAAA1850.058.783781
GCAGGGA651.0665863E-555.769234
AAGCAGG2100.055.2380946
GGTCTGC400.005990548554.3751
TGCACGC450.00954337348.3333325
AAGAAGG450.00954337348.3333325
TACCTGC604.8635332E-448.3333323
CTGCACG450.00954337348.3333324
Produced by FastQC (version 0.11.5)