FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_CM551087_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_CM551087_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201481
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA20480.1704562951890209No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC17750.1477343378713438No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC14550.12110054174805926No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC13800.11485824578166447No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC12240.10187427017156327No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA28700.074.547891
GAGCGAA5950.074.3546451
GCAAAAG32350.065.898863
CGTCAGA350.003564505462.1524162
AGCAAAA34450.061.88182
CGACGGG606.801898E-660.423455
CAAAAGC35650.060.203114
CGAAAGC8450.059.2102933
ATTACGA651.0914349E-555.7894251
TACGACG651.0927815E-555.7778133
ACGACGG651.0930509E-555.775494
AGCGAAA9000.054.7976151
ACCGTGA400.006052481554.3720478
GCGAAAG9300.053.79862
CGTGTAG8500.052.035255
TTACGAC701.6946482E-551.793682
AAAGCAG49800.051.250736
TCACGCA1700.051.184352
TATCGTG8500.051.184352
GCGTCAG450.00962582848.3508341
Produced by FastQC (version 0.11.5)