FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_CM550443_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_CM550443_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2323568
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA26250.0107.2198641
GAGCGAA8450.088.420571
CGAAAGC32300.086.882383
GCGAAAG36800.077.048032
GGTCGTC300.00193637772.497067
GCAAAAG36950.038.464923
GAAAGCA74700.038.1492354
GAGCAAA36000.037.4785921
CTATTCG8550.037.308439
AAAGCAG113350.035.568635
GAGCGTT1051.8549112E-434.5224089
ACTATTC10150.034.2843258
AAGCAGG115050.032.206846
AGCAAAA41000.032.013072
CAGTACG1652.3889788E-630.7563299
TAAACTA11850.030.596065
CAAAAGC42750.030.5316474
ATTCGAC6400.029.4690551
TAGAAAC20950.029.4210453
TTCGACA6400.028.326482
Produced by FastQC (version 0.11.5)