FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_CM550292_2_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_CM550292_2_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632641
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA19010.11643711017915144No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT17290.105902032351264No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT16870.10332951334677985No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC16740.10253325746443952No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG16630.10185950248707462No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA16490.10100199615224659No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCG259.385046E-487.006475
GAGCGAA6450.076.470241
CGACGGG1350.075.190785
ACGACGG1350.075.190784
TACGACG1400.072.5098343
TTACGAC1500.067.675852
CGAAAGC10250.065.081993
AGCGAAA11000.060.0056271
ATTACGA1700.059.7341041
GCGAAAG11500.058.007872
AGCAAAA39700.053.6974562
GCAAAAG38900.053.6833723
GAGCAAA41150.052.8803941
CAAAAGC40450.052.69864
TATCGTG5150.049.2785262
CCGTCAT450.00964088748.332485145
CGTGTAG5550.045.724125
ATATCGT5900.043.028811
AAGCAGG60250.042.5967647
AAAAGCA55800.038.8514565
Produced by FastQC (version 0.11.5)