FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_CM550292_1_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_CM550292_1_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423821
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC9080.21424138964326922No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA8260.19489359894861275No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC5520.13024366418841918No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA5100.12033382017408294No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5030.1186821795050269No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC4950.11679459016896285No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC4880.1151429494999068No Hit
GAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTGCAGTGAGCTGA4680.11042397615974668No Hit
CACTCTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAG4330.10216577281446648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2400.0108.7947161
GGTCGTT259.3852653E-486.994697
GAGCAAA12550.079.753881
AGCAAAA13450.074.9299242
GCAAAAG13850.072.765883
CAAAAGC13950.072.755424
AGCGAAA4050.062.657892
CGAAAGC4050.062.6504974
CGTATAT350.00355928562.1684041
GACGACT350.003565948262.1390657
AGGTCGT350.003565948262.1390656
GCGAAAG4200.060.420113
CGTGTAG3150.057.536175
AAAAGCA17800.057.426275
AAAGCAG22200.057.147416
AAGCAGG22950.055.5957347
TATCGTG3300.054.9273722
TCGTGTA3400.053.305574
ATATCGT3400.051.1975061
ATCGTGT3600.050.350093
Produced by FastQC (version 0.11.5)