FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_BHHS1178_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_BHHS1178_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2096979
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT43960.2096349081225897No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG39020.1860772091661385No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA38350.1828821366356077No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT37830.1804023788507181No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG36000.1716755389538951No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC33880.16156575721549907No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC30960.14764096350034978No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT29730.1417753825860917No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA29400.140201690145681No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG28990.138246496507595No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT27910.1330962303389781No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC27420.13075953550321676No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC26230.12508470518779635No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG26190.12489395458895869No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA23780.1134012310089896No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGACTGCTTTCTTTGGCATATCC22040.10510357995955133No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG21580.10290994807291823No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA21190.10105012973425104No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG21110.10066862853657572No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC12600.078.854923
AGCGAAA14550.068.306291
GCGAAAG15200.065.36972
GAGCGAA7250.061.037251
GCAAAAG33900.057.3342173
TATCGTG2850.048.3513152
AGGGTGT10150.045.7236566
CGTGTAG3100.044.4445885
CGTACTC5100.041.2388533
TCGTACT5150.040.840432
CAAAAGC50450.039.9586334
TATATTC4600.039.4149483
GTACTGA5050.035.898446
AGCAAAA54850.035.7015152
CAAGGGT17150.035.5174834
TAGAAAC23250.035.249672
AAGCAGG70550.034.013747
ATATCGT4150.033.213041
AAGGGTG15150.032.5479245
ACAAGGT13100.031.5447448
Produced by FastQC (version 0.11.5)